Plasma-derived exosomal miR-34a-5p: a potential diagnostic biomarker for total colonic aganglionosis.

ABSTRACT:

Background

Hirschsprung's disease (HSCR) is marked by the absence of enteric neurons in the distal colon, with total colonic aganglionosis (TCA) representing a rare and severe variant of the condition. The objective of the study is to examine critical microRNAs and their corresponding genes in plasma-derived exosomes from patients with HSCR, focusing on their potential as biomarkers for TCA.

Methods

A total of nine individuals with HSCR, including three cases of TCA, were enrolled in the study between September 2019 and July 2022. Additionally, 10 healthy children were recruited as controls. Plasma-derived exosomes were isolated using ultracentrifugation, followed by total RNA extraction. The microRNAs within these exosomes were analyzed using small RNA next-generation seque

SUBMITTER: Wang J 

PROVIDER: S-EPMC12597202 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature