A Simplified Perchloric Acid Workflow With Neutralization (PCA N) for Democratizing Deep Plasma Proteomics at Population Scale.

ABSTRACT: Large-scale plasma proteomics studies offer tremendous potential for biomarker discovery but face significant challenges in balancing analytical depth, throughput, and cost-effectiveness. We present an optimized perchloric acid-based workflow with neutralization-PCA-N-that addresses these limitations. By introducing a neutralization step following protein precipitation, PCA-N enables direct enzymatic digestion without additional purification steps, reducing sample volume requirements to only 5 μl of plasma while maintaining deep plasma proteome coverage. The streamlined protocol allows preparation of over 10,000 samples per day using 384-well formats at costs comparable to undepleted plasma analysis (NEAT). Rigorous validation according to the recently introduced CLSI C64 guideline demonst

SUBMITTER: Albrecht V 

PROVIDER: S-EPMC12597270 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature