An in vitro evaluation of common botanical extracts on carboxylesterase 1 catalytic activity.

ABSTRACT: Carboxylesterase 1 (CES1) is an abundant hepatic drug metabolizing enzyme whose substrates include endogenous compounds and medications from a variety of therapeutic classes. CES1 is a serine hydrolase that catalyzes the cleavage of amides, esters, and thioesters. Concerns have been raised over botanical-drug interactions (BDIs) and their potential to impair enzymatic function resulting in therapeutic failures or adverse effects. BDIs have been extensively investigated with the cytochrome P450 family of enzymes; however, CES1 BDIs have remained largely uninvestigated. This study assessed the CES1 in vitro inhibitory effects of 18 commonly used botanical extracts and their major constituents using an established probe assay and liquid chromatography-tandem mass spectrometry analysis. The fo

SUBMITTER: Melchert PW 

PROVIDER: S-EPMC12597554 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature