Spatial transcriptomics reveals unique inflammatory signatures across all anatomic locations in postoperative Crohn's disease.

ABSTRACT:

Background and aims

Most patients with Crohn's disease (CD) who have undergone ileocolonic resection experience recurrent inflammation within 1 year after surgery. We examined the molecular basis underlying gastrointestinal inflammation in postoperative CD across 3 common anatomic locations of recurrence.

Methods

To characterize spatial transcriptomic signatures, this study utilized biopsies from the colon, neo-terminal ileum, and anastomosis of patients with postoperative CD in the PREDICT-OR study. Sample analyses were performed with 10X Genomics Visium CytAssist system V2.0, and data analyses with R.

Results

Histologically inflamed biopsies from all locations shared transcriptional signatures across 3 cellular niches (myeloid, B, T cells) and a specialized epitheli

SUBMITTER: Battat R 

PROVIDER: S-EPMC12597670 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature