Paternally multi-generational high-fat diet causes obesity and metabolic disorder through intergenerational DNA methylation.

ABSTRACT:

Objective

Paternal high-fat diet (HFD) has detrimental effects on offspring. However, the extent of comprehensive damage and the underlying mechanisms associated with sustained multigenerational HFD exposure remain unclear. This study aims to investigate intergenerational progressive accumulation of obesity and glycolipid metabolic disorders, as well as mechanisms mediated by DNA methylation.

Methods

We performed a novel paternally multi-generational HFD consumption model in male C57BL/6 J mice, while excluding maternal gestational effects and any confounding influences from females. The body weight and glycolipid metabolism indicators of each generation of male mice were determined. The intergenerational transmission of CpG methylation and gene expression variation was dete

SUBMITTER: Guo T 

PROVIDER: S-EPMC12597747 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature