In House Rapid, Simple Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA): A Reliable Tool for Enterobacter hormaechei Genotyping.

ABSTRACT: Enterobacter hormaechei, a prominent species within the Enterobacter cloacae complex, is a significant cause of nosocomial infections and is frequently associated with multidrug resistance. Rapid genomic comparison helps guide timely infection control measures. This study aimed to develop a simple and rapid multiple-locus variable-number tandem-repeat analysis (MLVA) protocol for epidemiological surveillance of E. hormaechei. Eight variable-number tandem-repeat (VNTR) regions were selected for amplification using multiplex PCR, followed by gel electrophoresis. The method's discriminatory power was evaluated on 46 unrelated strains from 22 French hospitals. Then, suspected related strains from three potential outbreaks, including ESBL- and/or NDM-producing isolates were compared. An indepen

SUBMITTER: Guillermard C 

PROVIDER: S-EPMC12597776 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature