Translation of the Morphological Hallmarks of Dyserythropoiesis to Objective Morphometric Parameters by Imaging Flow Cytometry.

ABSTRACT:

Introduction

Imaging flow cytometry (IFC) is a unique method combining multiparameter flow cytometry (MFC) with morphological evaluation of single cells. Since both analyses are integrated in the diagnostic work-up of myelodysplastic neoplasms (MDS), we wanted to explore the possibilities of IFC as a diagnostic tool for MDS, with focus on dyserythropoiesis.

Methods

We analysed fresh bone marrow (BM) aspirates from 26 patients with untreated MDS and MDS/MPN and compared them with 12 normal BM specimens (NBM) exploring the cytoplasmic compartment, nuclear abnormalities, and megaloblastoid changes.

Results

The cytoplasmic compartment in MDS showed higher contrast and variance values compared to NBM (p < 0.001). Cells with abnormal nuclei and binucleated forms were signif

SUBMITTER: Despoina Violidaki DV 

PROVIDER: S-EPMC12597861 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature