BIWT: a bioinformatics walkthrough for embedding spatial multiomics in agent-based models for virtual cells.

ABSTRACT:

Summary

Whereas transcriptomic and spatial profiling offer static snapshots of tissue structure, mechanistic models use biological rules to predict how tissues evolve. We present the BioInformatics WalkThrough (BIWT) software to directly initialize spatial agent-based models from single-cell and spatial molecular data. We demonstrate how initialization strategies affect tumor-immune dynamics and spatial clustering, positioning BIWT as a software suite to generate data-driven virtual cells representing both experimental and clinical contexts.

Availability and implementation

The BIWT software is available at https://github.com/PhysiCell-Tools/PhysiCell-Studio. The sample dataset for running the BIWT is available at https://zenodo.org/records/16365625. The code and instructions

SUBMITTER: Bergman DR 

PROVIDER: S-EPMC12597895 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature