Metabolic priming alters the morphology and metabolism of human dermal fibroblasts.

ABSTRACT: The metabolic environment provided by the culture medium plays a critical role in shaping cellular function and mitochondrial activity in vitro. In this study, we investigated the effects of metabolic priming on the metabolism and morphology of Normal Human Dermal Fibroblasts (NHDFs) by manipulating glucose availability in the culture medium. Our strategy involved transitioning NHDFs from traditional high-glucose medium (HGm) to either a medium with physiological glucose levels (LGm) or a glucose-free, galactose-containing medium (OXm). Prior to cellular characterization, we confirmed the absence of glucose in the culture media and fetal bovine serum using ¹H nuclear magnetic resonance (NMR) spectroscopy. Given previous observations of elevated reactive species under gluc

SUBMITTER: Pinho SA 

PROVIDER: S-EPMC12598107 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature