Establishment of a medial arterial calcification model in C57BL/6J mice via arterial intimal injury.

ABSTRACT:

Background

Medial arterial calcification (MAC) increases vascular stiffness and reduces arterial compliance, often leading to serious systemic vascular diseases. However, research progress in this field has been limited by the lack of effective animal models. To address this gap and facilitate MAC research, this study established a novel experimental animal model of MAC in wild-type C57BL/6J mice and developed corresponding pathological grading standards.

Methods

To establish an optimal MAC modeling protocol, we systematically compared key parameters, including wire diameter, modeling duration, and combination with a vitamin D₃ (VD₃) diet. The resulting model was then subjected to interventional treatments with various calcification inhibitors. For path

SUBMITTER: Hu C 

PROVIDER: S-EPMC12598240 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature