Deep-NIR to NIR-II hemicyanine fluorophore scaffolds with dual optically tunable sites for <i>in vivo</i> multiplexed imaging.

ABSTRACT: Dual-locked probes are highly promising for use in medical diagnosis and drug screening due to their enhanced specificity and multiplex detection capabilities. However, most near-infrared (NIR) fluorophores used for dual-locked systems exhibit spectral activity in the blue portion (650-800 nm) of the NIR window, substantially restricting their application in deep-tissue imaging. Herein, for the first time, we report a novel class of deep-NIR to NIR-II hemicyanine fluorophore (Guilin, GL) scaffolds with dual optically tunable sites for dual-locked probe development. These GL dyes, obtained through chloride-substituted cyanine/hemicyanine hybridization, exhibit emission wavelengths spanning 800-950 nm and contain critical meso-Cl and hydroxyl/amino groups, which can be readily convert

SUBMITTER: Liu Q 

PROVIDER: S-EPMC12598615 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature