Exploring Unspecific Peroxygenase Selectivity with Diverse Hydrocarbon Substrates.

ABSTRACT: Unspecific peroxygenases (UPOs) are enzymes capable of oxidising various substrates using hydrogen peroxide as a co-substrate. UPOs have gained interest due to their broad substrate specificity, which includes relatively inexpensive precursors for valuable compounds, such as pharmaceuticals and chemical building blocks. In this study, the activity of a panel of 30 UPOs toward aliphatic and aromatic hydrocarbons was screened to assess variation in substrate selectivity. Most of the studied UPOs were able to oxidise the substrates efficiently, producing 2-5 products via hydrogen abstraction or epoxidation. Overall trends were observed, such as the preference for aromatic oxidation over benzylic hydroxylation in the case of toluene, while the opposite was observed with ethylbenzene. Different

SUBMITTER: Rytkonen E 

PROVIDER: S-EPMC12598796 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature