TFNAs induce autophagy via activation of the nonclassical cGAS-STING signalling pathway to attenuate acute lung injury in mice.

ABSTRACT:

Background

Acute lung injury is a common and fatal inflammatory condition in critically ill patients. Tetrahedral framework nucleic acids (TFNAs) have good potential for treating inflammatory diseases. The aim of this study was to use TFNAs in the treatment of acute lung injury (ALI) in mice to investigate the effect and possible mechanism.

Method

The characteristics of the TFNAs, including particle size and cellular uptake, were detected. TFNAs were subsequently used to treat an ALI mouse lung epithelial cells (MLE12) model with or without an autophagy inhibitor. Flow cytometry and Western blotting (WB) were performed to detect apoptosis and autophagy. The oxidative stress level was assessed by measuring the malondialdehyde (MDA) content, superoxide dismutase (SOD) activity

SUBMITTER: Wei C 

PROVIDER: S-EPMC12598824 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature