Production of hydrogen from crude glycerol via an integrated process of L-malate biosynthesis by Escherichia coli and photofermentation by Rhodobacter capsulatus.

ABSTRACT: Biorefinery is an innovative concept for resource utilization. To further increase its efficiency, the integration of different biomass conversion technologies (integrated biorefinery) is essential. Biodiesel, a traditional biofuel, generates glycerol as an inevitable byproduct; making it an attractive carbon source for biorefineries due to its abundance, low price, and high degree of reduction. In this work, we propose an integrated two-step process of dark fermentation with Escherichia coli to produce L-malate from glycerol and photofermentation by Rhodobacter capsulatus to convert this L-malate into hydrogen, a clean and sustainable energy carrier. To this end, we optimized an E. coli L-malate-producing strain by overexpressing GlpK in the M4-Δiclr/pBAD-pck strain and scale up the proce

SUBMITTER: Marcos JL 

PROVIDER: S-EPMC12599024 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature