Single-cell immune landscape of acute kidney injury after cardiac surgery with cardiopulmonary bypass.

ABSTRACT:

Objectives

Acute kidney injury (AKI) occurs in 19-42% of patients with cardiac surgery under cardiopulmonary bypass (CPB). Clarifying their landscape of immune cells may help discover the mechanisms and thus the treatment and preventive strategies.

Methods

This prospective, single center study recruited adult patients scheduled for cardiac surgery. The primary outcome was AKI. Blood samples were harvested from the central vein for single-cell RNA sequencing, flow cytometry and protein microarrays to characterize the landscape of immune cells. Here we compared the single-cell RNA sequencing of 42,362 immune cells in the blood of 4 patients before and after cardiac surgery, depending on whether they experienced AKI or not.

Results

We observed an increased degree of stro

SUBMITTER: Wang T 

PROVIDER: S-EPMC12599361 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature