Cytokine responses to respiratory syncytial virus infection in well-differentiated infant nasal epithelial cell cultures.

ABSTRACT:

Purpose

Differentiated airway epithelial cell cultures closely mimic the in vivo airways. We compared the structure and cytokine response of well-differentiated human nasal epithelial (HNE) cells obtained from healthy infants (iHNE) and adults (aHNE).

Materials and methods

Human bronchial epithelial (HBE) progenitor (n=4) and primary HNE cells (n=4 aHNE; n=10 iHNE) were cultured using a modification of the air-liquid interface (ALI) approach. For differentiated HBE and HNE cell cultures, representative transwells were fixed and then stained for ciliated and goblet cells. Low (400 pfu) and high (10⁵ pfu) inoculums of recombinant green fluorescent protein-expressing respiratory syncytial virus (RSV) were added apically to the ALI cultures. The concentrations of cyto

SUBMITTER: Yun KW 

PROVIDER: S-EPMC12599452 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature