Sequence diversity within the HA-1 gene as detected by melting temperature assay without oligonucleotide probes.

ABSTRACT:

Background

The minor histocompatibility antigens (mHags) are self-peptides derived from common cellular proteins and presented by MHC class I and II molecules. Disparities in mHags are a potential risk for the development of graft-versus-host disease (GvHD) in the recipients of bone marrow from HLA-identical donors. Two alleles have been identified in the mHag HA-1. The correlation between mismatches of the mHag HA-1 and GvHD has been suggested and methods to facilitate large-scale testing were afterwards developed.

Methods

We used sequence specific primer (SSP) PCR and direct sequencing to detect HA-1 gene polymorphisms in a sample of 131 unrelated Italian subjects. We then set up a novel melting temperature (Tm) assay that may help identification of HA-1 alleles without ol

SUBMITTER: Graziano C 

PROVIDER: S-EPMC1260020 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature