Targeting cystatin F activation enhances NK cell cytotoxicity in glioblastoma models.

ABSTRACT:

Introduction

Glioblastoma (GBM) is a highly invasive brain tumor with limited treatment options and poor prognosis. Natural killer (NK) cells are key effectors of antitumor immunity, capable of eliminating cancer stem-like cells. However, GBM creates an immunosuppressive microenvironment that limits NK cell function. Here, we identify cystatin F as an immunosuppressive factor involved in regulating NK cell granule-mediated cytotoxicity.

Methods

We analyzed cystatin F expression in GBM and its correlation with immune exhaustion markers. NK cell activity was compared between GBM patients and healthy donors. In vitro co-cultures of cystatin F-expressing microglial cells and glioblastoma stem-like cells were used to assess NK cell function. To block cystatin F activation

SUBMITTER: Senjor E 

PROVIDER: S-EPMC12602517 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature