The synaptonemal complex component corolla regulates meiotic crossover formation in Drosophila melanogaster.

ABSTRACT: In Drosophila melanogaster females, as in most organisms, the segregation of homologous chromosomes in meiosis depends on the formation of crossovers between them. In most cases, crossovers require the synaptonemal complex (SC), a conserved multi-protein structure that forms between homologous chromosomes in early meiosis. Recent studies leveraging partial-loss-of-function alleles suggest that the SC plays a more direct role in crossover formation. One SC protein that is involved in crossover formation is SYP-4 in nematodes, which we found is a likely ortholog of the D. melanogaster SC protein Corolla. To create a hypomorphic allele of corolla in D. melanogaster, we used CRISPR/Cas9 to replace it with its D. mauritiana ortholog, yielding corolla^mau. Since SC protein sequences ar

SUBMITTER: Williams S 

PROVIDER: S-EPMC12602556 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature