Non-standardized protein background in IVF media linked to serum-derived albumin supplementation.

ABSTRACT:

Purpose

To explore the protein compositional variability of IVF media and identify sources of undeclared contaminants that interfere with the detection of embryo-derived signals.

Methods

Untargeted and targeted mass spectrometry techniques were used to analyze protein composition in 85 samples of used and unused monophasic IVF media across 13 production lots from two manufacturers. Samples included spent culture media (SCM) from individual embryo cultures, matched controls, and unused (blank) media. Protein-free base media was supplemented with either serum-derived or recombinant human serum albumin (HSA) to evaluate their impact on protein contamination.

Results

Proteomic analysis revealed that not only SCM but also unconditioned media contained over 700 undeclared h

SUBMITTER: Nezvedova M 

PROVIDER: S-EPMC12602788 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature