Lipidomic profiling of human bile distinguishes cholangiocarcinoma from benign bile duct diseases with high specificity and sensitivity: a prospective descriptive study.

ABSTRACT:

Background

Cholangiocarcinoma (CCA) is a rare and highly aggressive malignancy originating in the bile ducts. Owing to limitations involving pathological sampling, the clinical differentiation of CCA from benign biliary diseases remains challenging. This study aimed to evaluate the differences between the bile lipidomes of CCA patients and those of patients with benign disease to develop a bile lipid classifier that can help to differentiate CCA from benign conditions.

Methods

Bile samples were collected by endoscopic retrograde cholangiography (ERCP) from patients with CCA or benign disease. The participants were divided into three cohorts: the first two cohorts underwent untargeted lipidomic analysis, whereas the third cohort was subjected to targeted lipid quantification.

SUBMITTER: Liu FS 

PROVIDER: S-EPMC12603035 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature