Neutrophil-derived Il1r2 modulates inflammation and alleviates acute lung injury by promoting M2 macrophage polarization.

ABSTRACT: Acute Lung Injury (ALI) and Acute Respiratory Distress Syndrome (ARDS) are severe inflammatory conditions with high morbidity and mortality. Understanding the molecular mechanisms underlying these diseases is crucial for developing effective treatments. To investigate the molecular mechanisms underlying ALI, we established a lipopolysaccharide (LPS)-induced mouse model. Bioinformatics and machine learning techniques were utilized to identify key genes and construct gene co-expression networks. Single-cell RNA sequencing was performed to analyze Il1r2 expression specifically in neutrophils. CellChat and hdWGCNA were employed to explore gene co-expression modules and cell-cell communication networks, respectively. Experimental validations included qRT-PCR for gene expression quantification,

SUBMITTER: Ding W 

PROVIDER: S-EPMC12603179 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature