Regulation of cellular states via targeted phosphorylation of p53 using a nanobody-coupled kinase system.

ABSTRACT: Phosphorylation participates in numerous signal transduction processes, including proliferation, differentiation, apoptosis, and cellular response to stimuli. Understanding its regulatory mechanisms is essential for advancing therapeutic interventions. In this study, we developed a target protein phosphorylation (TPP) system, consisting of a nanobody fused to a kinase domain, to investigate its ability to phosphorylate target proteins and regulate their cellular characteristics. We first verified that the nanobody-coupled kinase effectively phosphorylates GFP. Subsequently, we focused on p53 phosphorylation, identifying specific phosphorylation sites targeted by the system. This phosphorylation resulted in stabilization of p53 protein levels, inducing p21 expression, delaying cell cycle pr

SUBMITTER: Lim HE 

PROVIDER: S-EPMC12603232 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature