<i>NEAT</i> and tidy: a novel RT-PCR method for the independent quantification of the <i>NEAT1_1</i> isoform.

ABSTRACT: The long noncoding RNA NEAT1 is transcribed from a single exon gene and produces two isoforms through alternative 3'-end processing. The short polyadenylated NEAT1_1 drives proliferation in many malignancies through increasing glycolytic flux and the Warburg effect. The longer NEAT1_2 lacks a poly(A)-tail but is an essential scaffold for nuclear paraspeckles, nuclear condensates that reportedly play a tumour protective role. Due to the two isoforms sharing identical 5'-ends, many previous studies have quantified NEAT1_1 by subtracting NEAT1_2 from total NEAT1 levels. However, this only estimates the abundance of NEAT1_1. Standard oligo(dT)-primed RT-PCR is not suitable for quantifying NEAT1_1 as the longer NEAT1_2 sequence cont

SUBMITTER: Smith NE 

PROVIDER: S-EPMC12603357 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature