Sustainable PUFA-Rich Lipid-Accumulating Biomass Production via Dual Waste Valorization Using Heterotrophic Microalgae Cultivated on Anaerobic Effluent and Molasses.

ABSTRACT: The growing global demand for sustainable solutions necessitates innovative strategies for biomass and high-value compound production from waste. This study explores the heterotrophic cultivation of microalga Chlorella sp. G049 using anaerobic effluent (AE) derived from food waste and molasses as cost-effective substrates to produce polyunsaturated fatty acid (PUFA)-rich lipids while concurrently treating wastewater. Initially, one-factor-at-a-time (OFAT) experiments identified 12.5% AE and 20 g/l molasses as effective concentrations for promoting growth. Subsequently, Response Surface Methodology (RSM) was employed to optimize co-utilization of AE and molasses, maximizing biomass concentration, lipid yield, and PUFA content. The optimal conditions-18.5% AE and 18.18 g/l molasses-re

SUBMITTER: Maneechote W 

PROVIDER: S-EPMC12603369 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature