Microbiome Profiling of Pretreated Human Breast Milk Using Shotgun Metagenomic Sequencing.

ABSTRACT: This study explored the metagenomic sequencing methodology for analyzing the breast milk microbiome and elucidated its composition. Twenty-two breast milk samples were collected from 11 healthy lactating women. By optimizing microbial cell wall disruption parameters and developing a nucleic acid extraction method, microbial DNA/RNA libraries were constructed and subjected to metagenomic next-generation sequencing (mNGS), microbial standards spiked into breast milk at serial dilutions served to validate the method's reliability. The sequencing data underwent rigorous quality control and classification using the Kraken2 software and a self-generated database. The breast milk microbiome was found to comprise 21 phyla, 234 genera, and 487 species, with Firmicutes and Proteobacteria</

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC12603376 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature