Single-cell multi-omics and spatial transcriptomics reveal the transcriptional regulatory landscape of clear cell renal cell carcinoma.

ABSTRACT:

Background

Clear cell renal cell carcinoma (ccRCC) represents the most aggressive form of renal cell carcinoma (RCC), distinguished by pronounced intratumoral heterogeneity, extensive metabolic reprogramming, and marked resistance to conventional therapeutic approaches. This study aimed to comprehensively characterize the cellular heterogeneity, epigenetic regulation, and transcription factor (TF) networks in ccRCC by integrating multi-omics data, and to identify functional key genes with prognostic and therapeutic significance.

Methods

Single-cell RNA sequencing (scRNA-seq), single-cell assay for transposase-accessible chromatin using sequencing (scATAC-seq), and spatial transcriptomics (ST) were integrated to comprehensively explore cellular heterogeneity, epigenetic regul

SUBMITTER: Duan J 

PROVIDER: S-EPMC12603821 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature