Two-Photon Fabrication of Donor-Specific Human Lamina Cribrosa Models.

ABSTRACT:

Purpose

The lamina cribrosa (LC) is a complex network of collagenous beams that maintains mechanical homeostasis in response to fluctuations in intraocular pressure, thus protecting retinal ganglion cell axons exiting the eye. Understanding the structure and function of the LC may provide new insights into glaucomatous neurodegeneration. The purpose of this study is to utilize a two-photon fabrication technique to fabricate a model of the human LC (mLC) to scale.

Methods

Segmented multiphoton microscopy images of human LC tissues from a prior study were used to two-photon polymerize three mLCs. The mLCs were subsequently imaged using micro-computed tomography. Regional and full-field structural anisotropies and global microstructure were compared between the input and micro-

SUBMITTER: Shittu RJ 

PROVIDER: S-EPMC12603958 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature