Comparative transcriptomic signatures of virulent and attenuated <i>Mycobacterium bovis</i> growing <i>in vitro</i> and in mice.

ABSTRACT: Bovine tuberculosis (bTB), caused by Mycobacterium bovis (M. bovis), poses a significant global health and economic burden. Despite extensive research, a comprehensive understanding of M. bovis pathogenesis, particularly its transcriptional adaptation across different growth phases and within the host environment, remains incomplete. Here, we performed a comprehensive transcriptomic analysis of virulent M. bovis and the attenuated M. bovis BCG strain (BCG) across early-log, mid-log, and stationary growth phases to elucidate the molecular underpinnings of their phenotypic distinctions. Differential expression was computed with DESeq2, and coexpression modules were derived with WGCNA. Gene sets emphasized secretion systems and lipid metabolism. For biologic

SUBMITTER: Abdelaal HFM 

PROVIDER: S-EPMC12604023 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature