Transcriptomics and Neuropeptidomics of the Cockroach <i>Rhyparobia maderae</i> to Characterize the Neuropeptide Landscape of the Cockroach's Circadian Clock.

ABSTRACT: The circadian clock of Rhyparobia maderae is the accessory medulla (AME) in the optic lobes of the brain. Controlled by compound ocular photoreceptors, the cockroach clock orchestrates circadian rhythms in physiology and behavior synchronized with environmental light-dark cycles. Approximately 240 neurons, rich in partially colocalized neuropeptides, innervate the AME. Since the identity and function of most of these neuropeptides are unknown in the cockroach, the transcriptome of the Madeira cockroach was prepared, revealing 68 neuropeptides, neuropeptide-like and protein hormone genes. To identify the neuropeptidome, a combination of analyses of brain/retrocerebral complex extracts by Q-Exactive Orbitrap mass spectrometry (MS) and direct tissue profiling of different parts of the

SUBMITTER: Neupert S 

PROVIDER: S-EPMC12604039 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature