Analysis of the Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Interactome.

ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) is a major swine pathogen that causes significant economic losses worldwide. The nucleocapsid (N) protein, the most abundant viral protein in infected cells, plays roles beyond its structural function, influencing various host cellular processes. Here, we report the identification of 301 cellular protein candidates interacting with PRRSV N using EGFP immunoprecipitation combined with label-free quantitative mass spectrometry. The analysis underscores the versatile nature of the N protein in targeting a wide range of cellular proteins and processes across multiple subcellular compartments. We observed strong enrichment of ribosomal proteins, nucleolar proteins involved in ribosome biogenesis, splicing factors, RNA helicases, and DN

SUBMITTER: Kovanich D 

PROVIDER: S-EPMC12604043 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature