Immunoproteomic Identification of <i>Scedosporium boydii</i> Antigens with Potential Diagnostic Interest in Cystic Fibrosis Patients.

ABSTRACT: Fungal infections caused by Scedosporium/Lomentospora species are a significant threat to patients with cystic fibrosis (pwCF), ranking as the second most common filamentous fungi in their airways after Aspergillus. Current serodiagnostic methods, such as counterimmunoelectrophoresis and ELISA using crude extracts, lack standardization and commercial availability and often show cross-reactivity with other fungi. This study aimed to identify specific Scedosporium boydii antigens with potential for serodiagnosis in pwCF. An immunoproteomic approach combining two-dimensional polyacrylamide gel electrophoresis, Western blot, and mass spectrometry was used to analyze the antigenic profile of S. boydii against sera from pwCF with positive cultures for Scedos

SUBMITTER: Martin-Souto L 

PROVIDER: S-EPMC12604049 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature