Miniaturization of a colorimetric cellulase activity assay on a microfluidic sensor platform.

ABSTRACT: Enzymes are important commercial products with widespread uses as industrial catalysts, processing aids and analytical reagents. Biological activity is the major critical quality attribute monitored during the enzyme production. Assaying the enzyme activity often requires laborious procedures that are challenging to implement on microfluidic chips for facile on-site use in hand-held mobile devices. Here, we show the integration of a colorimetric assay of cellulase activity into a prototype microfluidic sensor platform on cyclic olefin copolymer foil obtained by roll-to-roll processing. The activity assay was based on resorufin dye release from the corresponding β-cellobioside substrate. Low water solubility of the substrate necessitated a concentration co-optimization for substrate and dim

SUBMITTER: Hengge E 

PROVIDER: S-EPMC12604091 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature