Development of two indirect ELISAs using purified recombinant viral proteins to detect myxoma virus-antigen specific antibodies in rabbit sera.

ABSTRACT: Despite the use of effective vaccines, myxomatosis remains a blight on European rabbit farms. The causative agent, myxoma virus (MYXV), is endemic in wild populations of leporids on the Iberian Peninsula. The detection of specific anti-MYXV antibodies in rabbit sera provides an essential part of control and epidemiological programs in both wild and domestic rabbits. Currently, the detection of specific antibodies is carried out by indirect (i)ELISA and competitive (c)ELISA, both using complete MYXV as antigen which requires stringent biosecurity controls. In this study, two full-length recombinant (r) viral proteins, rM022 and rM115, were shown to be antigenic and fit for the development of independent iELISAs for the detection of anti-MYXV antibodies in rabbit sera. The two iELISAs were o

SUBMITTER: Calonge-Sanz I 

PROVIDER: S-EPMC12604247 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature