Truncated APC impairs innate immune response by targeting MAVS on mitochondria in colorectal cancer.

ABSTRACT:

Background

Most mutant adenomatous polyposis coli (APC) gene produced truncated APC protein (Trunc-APC), which has been shown to function as an oncogene in colorectal cancer (CRC) pathogenesis; however, its role in modulating innate immune responses within tumor cells remains unexplored.

Methods

We utilized CRISPR-Cas9 to knockout mutant APC and performed transcriptome sequencing across multiple CRC cell lines to investigate the immunomodulatory function of Trunc-APC. Subcellular fractionation, proteinase K protection assays, and immunofluorescence were employed to determine Trunc-APC subcellular localization. Protein interaction studies, ubiquitination assays, and aggregation analyses were conducted to elucidate Trunc-APC binding to MAVS and its impact on MAVS ubiquitinatio

SUBMITTER: Li SY 

PROVIDER: S-EPMC12604278 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature