Decoding the microbial assembly and environmental drivers along the phyllosphere-rhizosphere continuum of leguminous green manure Astragalus sinicus.

ABSTRACT: Green manure crops are increasingly recognized not only for their contributions to soil fertility but also for their role in shaping plant-associated microbiome. Astragalus sinicus, a widely used leguminous green manure in East Asian paddy fields, harbors distinct microbial assemblages across plant compartments, yet the ecological processes driving microbiome assembly along its phyllosphere-rhizosphere continuum remain unclear. In this study, we profiled microbiome composition across the rhizospheric, phyllospheric, and soil compartments of A. sinicus using 16S rRNA gene amplicon sequencing targeting the region south of the Yangtze River, analyzing 315 samples collected from seven rice-growing regions. We found that Proteobacteria predominated all sampled compartments, with Mesorhizobium (

SUBMITTER: Huang Y 

PROVIDER: S-EPMC12604385 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature