A new piece in the repeatome puzzle of Triatominae bugs: The analysis of Triatoma rubrofasciata reveals the role of satellite DNAs in the karyotypic evolution of distinct lineages.

ABSTRACT: The genome of Triatoma rubrofasciata, a representative of the North American Triatomini lineage, was analysed to characterise its repetitive DNA content and satellite DNA (satDNA) organisation. Using RepeatExplorer2, we determined that repetitive elements comprise approximately 25% of the genome in a male sample from Vietnam and 16% in a female sample from China, with satDNA being the most abundant component. The satellitome analysis revealed 126 satDNA families in the male and 114 in the female, with marked quantitative differences driven by the amplification of two satDNA families: TrubSat001-166 and TrubSat002-9. Fluorescence in situ hybridization (FISH) confirmed that TrubSat002-9 is enriched in the Y chromosome, explaining its lesser abundance in the female genome. Chromosomal mapping

SUBMITTER: Pita S 

PROVIDER: S-EPMC12604443 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature