Extracellular matrix stiffness regulates the proliferation and migration capacities of lymphatic endothelial cells via FAT1.

ABSTRACT:

Introduction

The extracellular matrix (ECM) stiffness serves as a critical biomechanical regulator of cellular behavior. However, its specific roles on lymphatic endothelial cells (LECs) remains poorly characterized, particularly in the context of tumorigenesis where progressive matrix stiffening is a hallmark of the tumor microenvironments (TME).

Methods

The effects of ECM stiffness on LEC proliferation and migration were assessed using a tunable polyacrylamide hydrogel system. Differential gene expression in LECs on soft versus stiff substrates was identified by RNA-seq. To evaluate the stiffness-dependent regulation of FAT1 and its downstream mechanisms, we performed RT-qPCR, Western blot, immunofluorescence, wound healing, and spheroid assays. Furthermore, immunofluoresc

SUBMITTER: Xu Z 

PROVIDER: S-EPMC12604528 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature