Comparison of immunometabolic profiles in whole blood versus peripheral blood mononuclear cells.

ABSTRACT:

Background

Immunometabolism has emerged as a flourishing field exploring how cellular metabolism regulates immune responses. Peripheral blood mononuclear cells (PBMCs) have so far been the primary sample type used for immunometabolic profiling. However, PBMCs isolation requires large blood volumes, can pose logistic challenges, and requires specialized skills for processing. Thus, using whole blood (WB) samples, which are less technically challenging to process, could serve as a viable alternative for metabolic characterization of circulating immune cell populations. Yet, how well WB immunometabolic profiles match those from PBMCs remains unknown. Therefore, we aimed to compare the immunometabolic profile of WB with that of PBMCs.

Method

Paired WB and PBMCs samples were coll

SUBMITTER: Diallo I 

PROVIDER: S-EPMC12604536 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature