Variation and spread of resistomes in swine manure, manure slurries, and long-term manure-fertilized soils.

ABSTRACT:

Background

Application of swine manure to soils exacerbates environmental antimicrobial resistance (AMR). However, a comprehensive evaluation of anaerobic digestion's (AD) mitigation potential against AMR and its influencing factors in swine manure-to-soil systems remains lacking.

Methods

We employed mass spectrometry, metagenomics, and whole-genome sequencing (WGS) to investigate the fate of antibiotics, metals, and antibiotic resistance genes (ARGs) across manures, slurries, and soils from eight pig farms.

Results

Anaerobic digestion reduced antibiotic and metal (except ciprofloxacin) content and risks in manure, but had limited effects on total ARG abundance, while increasing ARG network modularity. High-risk ARG abundance significantly increased from 404.7 in manu

SUBMITTER: Jin L 

PROVIDER: S-EPMC12605092 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature