Rapid identification of <i>Acinetobacter baumannii</i> using novel specific molecular targets derived from pan-genome analysis and its clinical application.

ABSTRACT: Acinetobacter baumannii is a pathogen capable of causing severe hospital-acquired infections such as ventilator-associated pneumonia and bacteremia, accounting for over 80% of nosocomial infections. Current nucleic acid tests (NATs) for A. baumannii suffer from limitations in specificity and sensitivity due to reliance on suboptimal targets. Therefore, this study aimed to identify novel, highly specific molecular targets for A. baumannii NATs using pan-genome analysis. A total of 9 specific molecular targets for A. baumannii were screened from 642 genome sequences by pan-genome analysis: outO, ureE, rplY, bioF, menH_3, hemW, paaF_1, smpB and ppaX. These specific species targets have been verified by BLAST and PCR of non-target strains to have 100% specifi

SUBMITTER: Chen S 

PROVIDER: S-EPMC12605139 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature