Single-cell RNA profiling reveals an immunosuppressive microenvironment in <i>EGFR</i> double-mutant non-small cell lung cancer.

ABSTRACT:

Background

Non-small cell lung cancer (NSCLC) patients with double-driver gene mutations are reported with poorer survival outcomes and reduced therapy responses compared to single-mutant (SM) patients. While substantial progress has been made in treating cancers with single-driver gene alterations, the therapeutic implications and tumor microenvironment of double-mutant (DM) NSCLC remain largely unexplored because of its rarity and poor prognosis. This study aims to delineate the landscape of the immune microenvironment within DM NSCLC.

Methods

We employed single-cell RNA sequencing (scRNA-seq) to generate a comprehensive transcriptomic atlas from 25 EGFR mutant NSCLC samples. To assess immune microenvironment changes over time, time-series scRNA-seq and multiplex im

SUBMITTER: Yang J 

PROVIDER: S-EPMC12605333 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature