MiRNA changes with ageing and caloric restriction in male rat skeletal muscle: potential roles in muscle cell function.

ABSTRACT: The mechanisms underlying skeletal muscle ageing, whilst poorly understood, are thought to involve dysregulated micro (mi)RNA expression. Using young and aged rat skeletal muscle tissue, we applied high-throughput RNA sequencing to comprehensively study alterations in miRNA expression occurring with age, as well as the impact of caloric restriction (CR) on these changes. Furthermore, the function of the proteins targeted by these age- and CR-associated miRNAs was ascertained. Numerous known and novel age-associated miRNAs were identified of which CR normalised > 35% to youthful levels. Our results suggest miRNAs upregulated with age to downregulate proteins involved in muscle tissue development and metabolism, as well as longevity pathways, such as AMPK and autophagy. Furthermore, our resu

SUBMITTER: Altab G 

PROVIDER: S-EPMC12605627 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature