CST7⁺ macrophages/monocytes in melanoma: single-cell insights into immunotherapy response.

ABSTRACT:

Background

Advanced melanoma exhibits high primary resistance to immunotherapy and limited long-term response, suggesting the need for sensitive biomarkers and novel therapeutic targets to enhance treatment efficacy. We aimed to identify prognostic biomarkers and therapeutic targets for melanoma immunotherapy.

Methods

Bulk RNA-sequencing data from public melanoma cohorts were analyzed to classify tumor samples into two clusters based on distinct expression patterns of immune-related hallmarks. Differential gene expression and least absolute shrinkage and selection operator (LASSO) Cox regression analysis identified nine core genes, which were integrated into a prognostic risk model. Single-cell RNA-sequencing analysis was then performed to characterize the immune cell hetero

SUBMITTER: Yao K 

PROVIDER: S-EPMC12605685 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature