Establishment, Characterization, and Cryopreservation of Feather Follicle Fibroblast Lines From Hyacinth Macaw (Anodorhynchus hyacinthinus).

ABSTRACT: Cryopreservation, biobanking, and in vitro propagation of cells from endangered species represent key strategies for advancing biodiversity conservation. The Hyacinth Macaw (Anodorhynchus hyacinthinus), a flagship avian species of the Brazilian Pantanal, is among the critically vulnerable taxa that stand to benefit significantly from such cutting-edge biotechnological interventions. This study developed and validated minimally invasive methods for isolating, culturing, and cryopreserving fibroblasts derived from feather follicles (FFFs) of Hyacinth Macaws. Cells isolated from nestlings under 100 days of age demonstrated superior yields and viability compared to older birds. Two cryopreservation media were tested-Cryomedium 1 (55% DMEM-F12, 35% FBS, 10% DMSO) and Cryomedium 2 (90% FBS, 10%

SUBMITTER: Nogueira IPM 

PROVIDER: S-EPMC12605831 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature