Comprehensive Analysis of the PANoptosis-Related Genes in Stroke Based on Single-Cell RNA-Seq and Spatial Transcriptomics.

ABSTRACT:

Background

PANoptosis is implicated in immunoinflammatory diseases, but its role in cerebral ischemia-reperfusion injury (CIRI) remains unclear.

Methods

We integrated single-cell RNA sequencing (scRNA-seq), spatial transcriptomics (ST), and bulk RNA-sequencing (bulk RNA-seq) datasets to explore PANoptosis-related molecular signatures in ischemic stroke. scRNA-seq identified cellular subpopulations; ST revealed spatial expression. Multimodal intersection analysis (MIA) and high-dimensional weighted gene coexpression network analysis (hdWGCNA) detected PANoptosis-related differently expressed genes (DEGs). Gene Set Enrichment Analysis (GSEA)-identified biomarkers were validated in GSE35338 and GSE137482. Analyses characterized spatiotemporal microglial dynamics. TNFRSF1A

SUBMITTER: Bai W 

PROVIDER: S-EPMC12605869 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature