Phytochemical Profile and α-Amylase Inhibitory Activity of Pomegranate Peel Extract: An In Silico and In Vitro Investigation.

ABSTRACT: Diabetes is among the top ten causes of mortality and morbidity. Diabetes has been increasing faster in low- and middle-income nations than it has been rising in high-income countries. This study explores the phytochemical profiling and in vitro α-amylase inhibitory activity of the methanolic extract of Nepalese-origin pomegranate peels. Phytochemical analysis using FT-IR and LC-MS identified key bioactive compounds, including ellagic acid, punicalagin, punicalin, gallic acid, and ellagic acid-O-xylopyranoside, contributing to the observed enzymatic inhibition. Molecular docking studies revealed strong binding affinities of punicalagin (-11.6 kcal/mol) and ellagic acid-O-xylopyranoside (-10.6 kcal/mol) to α-amylase, suggesting potential antidiabetic properties. MD simulations confirmed the

SUBMITTER: Thapa S 

PROVIDER: S-EPMC12605963 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature