The BHMT2/MAT1A/AHSG axis promotes M1 macrophage activation and exacerbates necrotizing enterocolitis.

ABSTRACT: Background Necrotizing enterocolitis (NEC) is a devastating intestinal disorder in premature infants, characterized by inflammation and tissue injury. Identifying key regulatory pathways contributing to NEC pathogenesis is essential for developing targeted therapeutic strategies. Methods Transcriptomic analysis of NEC and control samples identified a core regulatory module comprising AHSG, BHMT2, and MAT1A. Their expression and functional roles were investigated in human primary intestinal epithelial cells (HPIECs), a transwell co-culture system with THP-1 macrophages, and a mouse model of NEC. Molecular techniques, including RT-qPCR, Western blotting, ELISA, chromatin immunoprecipitation, and flow cytometry were employed to decipher the functional mechanism of this regulatory module. Resu

SUBMITTER: Yue W 

PROVIDER: S-EPMC12606173 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature