Residue-Based Thermogravimetric Analysis: A Novel Method to Quantify Carboxylate Group Modifications in Macromolecules.

ABSTRACT: Quantifying the degree of modification (DoM) of hyaluronic acid (HA) is crucial for biomaterials development. This has remained a challenge, as diverse functional groups hinder precise spectroscopic quantification. Here, we present a method employing thermogravimetric analysis (TGA) by comparing residues of sodium hyaluronate (NaHA) and carboxylate-modified HA derivatives. Thermal decomposition enabled quantification of the inorganic residue (Na₂CO₃) that was obtained as the final product. Validation on four diverse HA derivatives, namely aldehyde, furan, thiol, and cyanoacetate, was performed. The first three matched ¹H NMR/UV-vis data, while the cyanoacetate sample, previously unquantifiable, was determined for the first time. Because the residue arises s

SUBMITTER: Leliopoulos C 

PROVIDER: S-EPMC12606623 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature