Lipidomics Techniques Revealed the Adipogenic Differentiation Mechanism of Bovine Adipose-Derived Neural Crest Stem Cells.

ABSTRACT: Bovine adipose-derived neural crest stem cells (baNCSCs) are an ideal model for studying the mechanism of adipogenesis. Lipidomics provides a powerful technical means to comprehensively analyze the dynamic changes in lipid metabolism during cell differentiation. However, the lipidomic remodeling throughout the adipogenic differentiation of baNCSCs is still lacking in-depth research. This study used ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to perform non-targeted lipidomic analysis on baNCSCs on day 0 (CON0) and day 9 (DIF9) of adipogenic induction and differentiation. Differential lipid metabolites were screened through multivariate statistical analysis and univariate analysis. A total of 1639 lipid molecules were identified. Compared with the CON

SUBMITTER: Zhang K 

PROVIDER: S-EPMC12606755 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature